Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
Component |
Type Strain |
Volume |
Biosafety Level |
Composition |
Product Format |
Storage |
Storage Conditions |
Lysis Buffer |
梅經(jīng)理 |
17280875617 |
1438578920 |
胡經(jīng)理 |
13345964880 |
2438244627 |
周經(jīng)理 |
17757487661 |
1296385441 |
于經(jīng)理 |
18067160830 |
2088210172 |
沈經(jīng)理 |
19548299266 |
2662369050 |
李經(jīng)理 |
13626845108 |
972239479 |
阿克苏市|
商河县|
嘉义市|
安仁县|
双牌县|
西青区|
北流市|
陇西县|
宕昌县|
互助|
扶余县|
济源市|
鸡泽县|
利辛县|
冷水江市|
龙口市|
锦州市|
涡阳县|
子长县|
新宁县|
浦东新区|
金湖县|
亳州市|
章丘市|
喀喇沁旗|
霞浦县|
金秀|
沙坪坝区|
明水县|
会泽县|
集安市|
新干县|
丽水市|
南京市|
武平县|
廊坊市|
安康市|
邢台县|
承德市|
瑞昌市|
澄迈县|
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