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Epizootic hemorrhagic disease virus 2
Epizootic hemorrhagic disease virus 2
規(guī)格:
貨期:
編號(hào):B162815
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Epizootic hemorrhagic disease virus 2
商品貨號(hào) B162815
Classification Sedoreovirinae, Orbivirus, Epizootic hemorrhagic disease virus
Agent Epizootic hemorrhagic disease virus 2
Strain OV215
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1897 images
Comments Strain fully sequenced by depositor
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero E6 cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor J Loeb, University of Florida
Chain of Custody ATCC <-- J Loeb
Source Spleen of a farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on October 11, 2016.
Year of Origin 2016
References

Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661

Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175

Cross References

Nucleotide (GenBank) : MF688816.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP1 protein (VP1) gene, complete cds.

Nucleotide (GenBank) : MF688817.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP2 protein (VP2) gene, complete cds.

Nucleotide (GenBank) : MF688818.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP3 protein (VP3) gene, complete cds.

Nucleotide (GenBank) : MF688819.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP4 protein (VP4) gene, complete cds.

Nucleotide (GenBank) : MF688820.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP5 protein (VP5) gene, complete cds.

Nucleotide (GenBank) : MF688821.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP6 protein (VP6) gene, complete cds.

Nucleotide (GenBank) : MF688822.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP7 protein (VP7) gene, complete cds.

Nucleotide (GenBank) : MF688823.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS1 protein (NS1) gene, complete cds.

Nucleotide (GenBank) : MF688824.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS2 protein (NS2) gene, complete cds.

Nucleotide (GenBank) : MF688825.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS3 protein (NS3) gene, complete cds.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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