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Nuclear polyhedrosis virus, Autographa californica
Nuclear polyhedrosis virus, Autographa californica
規(guī)格:
貨期:
編號:B163011
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Nuclear polyhedrosis virus, Autographa californica
商品貨號 B163011
Classification Baculovirus
Deposited As Nuclear Polyhedrosis Virus, Lymantria dispar (plaque purified)
Agent Nuclear polyhedrosis virus, Autographa californica
Strain LdMNPV-Ab-a624
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA
Product Format freeze-dried
Storage Conditions -20°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
To propagate, rehydrate in water (or thaw), and feed to gypsy moth larvae (and collect hemolymph), or dissolve in 0.1 M Na2CO3, 0.2 M NaCl (pH 10.8) and inoculate gypsy moth cell culture (and collect cell lysate and supernate).
Effect on Host
Yes, in vivo effects: Kills 4th-5th instar Lymantria dispar larvae, 5-7 days
Yes, in vitro effects: nuclear swelling and inclusion bodies in cell culture, 3-4 days
Recommended Host
gypsy moth larvae
gypsy moth larvae and closely related lymantrids; IPLB LDFB, IPLB 652Y, and IPLB EIT cell cultures
Growth Conditions
Temperature: 28°C
Incubation: 7 days 7 days
Name of Depositor M Shapiro
U.S. Patent
U.S. Patent Number
Source
derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA
References

Shapiro M, et al. More virulent biotype isolated from wild-type virus. US Patent 5,132,220 dated Jul 21 1992

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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