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15F7
15F7
規(guī)格:
貨期:
編號:B163587
品牌:Mingzhoubio

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產品名稱 15F7
商品貨號 B163587
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591).
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain Wistar (B cell); BALB/c (myeloma)
Applications
The antibody reacts with CD1.1, but cross-reacts with CD1.2 only at very high concentrations.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD1.1
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD1.1
Comments
Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected).
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody reacts with CD1.1, but cross-reacts with CD1.2 only at very high concentrations.
The antibody blocks recognition of CD1.1 by T cells in vitro.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Maintain cultures at cell concentrations between 2 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b; kappa light chain
Name of Depositor A Bendelac
Deposited As rat (B cell); mouse (myeloma)
References

Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266

Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267

Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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