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FRAN4
FRAN4
規(guī)格:
貨期:
編號:B164468
品牌:Mingzhoubio

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產(chǎn)品名稱 FRAN4
商品貨號 B164468
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? ? (B cell); BALB/c (myeloma)

Applications
The antibody is protective against tularemia in animal models using the LVS strain.
It cross-reacts with wild-type F. tularensis.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Francisella tularensis lipopolysaccharide
Cellular Products
immunoglobulin; monoclonal antibody; against Francisella tularensis lipopolysaccharide
Comments
Animals were immunized with live F. tularensis (LVS strain).
Spleen cells were fused with mouse myeloma cells.
The antibody is protective against tularemia in animal models using the LVS strain.
It cross-reacts with wild-type F. tularensis.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell density to exceed 1 X 10(6) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Name of Depositor WRAIR
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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