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KU812F [KU-812-F]
KU812F [KU-812-F]
規(guī)格:
貨期:
編號:B164929
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 KU812F [KU-812-F]
商品貨號 B164929
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type basophil
Product Format frozen
Morphology myeloblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease chronic myelogenous leukemia
Age 38 years adult
Gender male
Ethnicity Japanese
Storage Conditions liquid nitrogen vapor phase
Karyotype 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 [PubMed: 85212346]
Derivation

KU812F is a clone of KU812 (ATCC CRL-2099).

The parent KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia.

Genes Expressed

Transforming growth factor beta (TGF-beta) RefOkamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048 

Cellular Products Transforming growth factor beta (TGF-beta) [PubMed: 2698048]
Comments

The cells contain at least one Ph1 (Philadelphia) chromosome.

The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers. RefKishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 3 x 105 and 3 x 106 cells/mL. Do not allow the cell concentration to exceed 3 x 106 cells/mL.
Medium Renewal: Add fresh medium every two to three days (depending on cell density).
Cryopreservation

Complete growth medium supplemented with 5% (v/v).

 

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor K Kishi
References

Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439

Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048

KU812F cells are capable of continuous erythroid differentiation as evidenced by producion of glycophorin A, carbonic anhydrase and adult and fetal types of hemoglobin.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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