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L5178-R (LY-R)
L5178-R (LY-R)
規(guī)格:
貨期:
編號:B164946
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 L5178-R (LY-R)
商品貨號 B164946
Organism Mus musculus, mouse
Tissue thymus
Cell Type chemically induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain DBA/2
Applications
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tested and found negative for ectromelia virus.
Derivation
This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tumorigenic Yes
Effects
Yes, in DBA/2 mice
Comments
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
After 6 months to 3 years in culture, the line converts to an X-ray sensitive state.
Tested and found negative for ectromelia virus.
Complete Growth Medium Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10 4 cells/mL and maintain between 3 x 10 4 and 7 x 10 4 cells/mL.
Medium Renewal: Twice per week
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor JZ Beer
Deposited As Mus musculus
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312

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