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A 1.4 kb NcoI/SacI fragment has been subcloned into a mammalian expression vector and used to demonstrate function in kidney cell line 293.

Construct size (kb): 5.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SacI
Vector end: SacI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1B
Gene name: 5-hydroxytryptamine (serotonin) receptor 1B
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 q13
Type of DNA: genomic
Insert end: SacI
Insert end: SacI
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: M84986
Nucleotides 1-1912 of the insert correspond to
nucleotides 1-1912 of M84986.

Insert lengths(kb): 2.0

Gene product: 5-hydroxytryptamine (serotonin) receptor 1B [HTR1B]

Restriction digests of the clone give the following sizes (kb): BglII--4.8; HindIII--4.5, 0.26; NcoI/SalI--4.3, 0.78; SstI--2.85, 2.0; XhoI--4.8.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.89; BglI--0.86; BglII--0.09; HindIII--0.12; NcoI--0.59; NotI--0.04; PstI--0.05, 0.16, 0.69; PvuI--0.51; PvuII--0.44, 1.35; SmaI--1.37.

A 1.4 kb NcoI/SacI fragment has been subcloned into a mammalian expression vector and used to demonstrate function in kidney cell line 293.

Contains an intronless open reading frame of 1170 nt that begins at nt 592 of the sequence.

Enzymes not detecting polymorphism: BamHI BglI BglII EcoRI HindIII MspI PstI PvuII RsaI SstI TaqI XbaI.