| 產(chǎn)品名稱 |
pcDNAI/neo-11B1 |
| 商品貨號(hào) |
B217053 |
| Designations |
pcDNAI/neo-11B1 |
| Species |
Rattus norvegicus, rat |
| Depositors |
CE Gomez-Sanchez, M Zhou |
| Applications |
in another host, produces protein cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) |
| Vector |
Construct size (kb): 8.5 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.5 Gene product: cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) [CYP11B1] Target Gene: cytochrome P450, subfamily XIB, polypeptide 1 (steroid 11-beta-hydroxylase) |
| Insert Size (kb) |
1.5 |
| Media |
ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--7.0, 1.5; EcoRV--8.5; HindIII--8.5; PvuII--2.2, 1.5, 1.45 (doublet), 0.8, 0.65, 0.4, 0.2; XhoI--8.5. The insert contains the following restriction sites (nt from the 5' end): ApaI--230; PvuII--429, 1036; EcoRV--783; HincII--945. |
| References |
Zhou M, Gomez-Sanchez CE. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem. Biophys. Res. Commun. 194: 112-117, 1993. PubMed: 8333830
Celso E Gomez-Sanchez, personal communication
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