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Distribution host: Escherichia coli DH5

Size (kb): 6.2199997901916500

DESCRIPTION OF VECTOR:
Intact vector size: 6.220
Type of vector: plasmid
Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Construction: pRS314
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): TRP1, ->, 2976-3650
promoter: PADH, 4437-5165
MCS: NdeI..PstI, ->, 5661-5730
ribosome-binding site: GAL4, ->, 5166-5607
transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938

Vector: pCD.2 (plasmid)

Promoters: Promoter PADH

Construction: pRS314

Marker(s):crbR

Construct size (kb): 6.21999979019165

Features: marker(s): TRP1
promoter: PADH
replicon: ARS4
replicon: ColE1
MCS: NdeI..PstI
ribosome-binding site: GAL4
transcription terminator: ADC1 transcriptional terminator (TADH)
centromere: CEN6
coding sequence: bla

cloning vector

shuttle vector

vector component of two-hybrid screening system

Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2.

Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4' DNA binding domain. It has lower copy number than 2 micron based plasmids.

Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5' GATC overhangs in all three reading frames.

Temperature: 37.0°C