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phHK15-2
phHK15-2
規(guī)格:
貨期:
編號:B231390
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 phHK15-2
商品貨號 B231390
Designations phHK15-2
Species Homo sapiens, human
Depositors GI Bell
Vector
Construct size (kb): 6.46999979019165
Insert
DNA: cDNA
Insert lengths(kb): 3.200000047683716
Tissue: kidney
Gene product: hexokinase 1 [HK1]
Alleles: A2, A1
Insert Size (kb) 3.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3 (doublet); EcoRI/PstI--3.3, 2.7, 0.8; HindIII--4.2, 2.5; BamHI--3.4, 2.0, 1.0; PstI--6.6.
This clone encodes amino acids 92-917 and includes 765 bp of 3'-untranslated sequence.
The insert was derived from a 3.25 kb EcoRI fragment of lambdahHEX-15 (ATCC 61510).
Enzyme(s) not detecting polymorphism: BclI, BglI, BglII, EcoRI, MspI, PstI, RsaI, SphI, TaqI, XbaI in a panel of 14 unrelated individuals.
References

Nishi S, et al. HincII RFLP at the human hexokinase I (HK1) locus on chromosome 10. Nucleic Acids Res. 17: 7547, 1989. PubMed: 2571978

Nishi S, et al. Human hexokinase: sequences of amino- and carboxyl-terminal halves are homologous. Biochem. Biophys. Res. Commun. 157: 937-943, 1988. PubMed: 3207429

Graeme I Bell, personal communication

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