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p4D8-B [p4D8B]
p4D8-B [p4D8B]
規(guī)格:
貨期:
編號:B242746
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p4D8-B [p4D8B]
商品貨號 B242746
Designations p4D8-B [p4D8B]
Species Homo sapiens, human
Depositors RE Schnur
Applications
This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations.
Vector
Construct size (kb): 10.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 8.0
Tissue: lymphoblast GM1416 (48,XXXX) cell line
Gene product: DNA Segment, single copy [DXS98]
Alleles: A1, B1, B2, C1, C2, D2, D1, A2
Insert Size (kb) 8.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.4, 1.1; HindIII--4.2, 3.4, 3.1; BamHI--10.9; PstI--4.1, 3.5, 1.4, 1.0, 0.7; SalI/BamHI--8.0, 2.9.
DXS98 shows close linkage to FRAXA (theta = 0.10, LOD score = 17.31).
DXS98 is proximal to FRAXA by in situ hybridization.
Combined heterozygosity for the polymorphisms detected by 4D-8 (ATCC 57060, 57061), p4D8-B (ATCC 61572, 61573), and p4D8-IV (ATCC 61574, 61575) is 48%.
This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations.
References

Schnur RE, et al. New polymorphisms at the DXS98 locus and confirmation of its location proximal to FRAXA by in situ hybridization. Am. J. Hum. Genet. 44: 248-254, 1989. PubMed: 2563194

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